Determination of Polyphenols in Areca catechu by HPLC

Abstract

Abstract:

HPLC was used to determine polyphenols in Areca catechu. Polyphenols were extracted from betel nut with methanol, which were extracted with petroleum ether, ethyl acetate, and n-butanol, successively. Then, the extracts were concentrated and determined by HPLC after making constant volume using the mobile phase. The results showed that the kinds and quantities of polyphenols in mature young betel nut were fewer than in mature fruit, while those in nuts of mature fruit were more than in the skin. The kinds and quantities of polyphenols in the methanol extract were the most. The content of catechin was the highest reaching 1610mg/kg in the polyphenols, tannic acid (622 mg/kg), epigallocatechin (228 mg/kg), epicatechin (164 mg/kg), epigallocatechin gallate (72 mg/kg), and gallic acid (13.6 mg/kg) were followed. However, catechin gallate, chlorogenic acid, and gallocatechin gallate were not detected.

Key words: HPLC, Areca catechu, polyphenols

WANG Ming-Yue, LUO Jin-Hui, LI Jian-Guo. Determination of Polyphenols in Areca catechu by HPLC[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(1): 101-104.

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