Flavonoids of Rhizoma Sparaganii Induce S/G2 Stage Arrest in A549 and MCF-7 Cells

Abstract

Abstract:

To investigate the cytotoxicity effects and mechanisms on estrogen receptor positive (ER⁺) cancer cell lines A549 and MCF-7 of the Rhizoma Sparganii flavonoids (RSF)，A549 or MCF-7 cells in DMEM mediums with 188，375，and 750 µg/mL RSF were cultured for 3 days，respectively. DMEM with 1% methanol culture cells as control. The cell proliferation activity，anti-a-tublin form，apoptosis as well as cell cycle differences between groups were studied by MTT，immunofluorescence，and flow cytometry technology. As a result，compare to the 1% methanol control groups，RSF could inhibit the proliferation activity，increase the rate of apoptotic bodies，and induce cytoskeleton disorder of the A549 and MCF-7 cells with the dose-related. In addition，S/G₂stage arrests were induced obviously in A549 and MCF-7 cells cultured with 375 and 750 µg/mL RSF. The results indicated that RSF showed a notable cytotoxicity against A549 and MCF-7 cells，and the inhibitions of cell proliferation were related with S/G2 cell cycle arrests.

Key words: Rhizoma Sparganii, flavonoid glycosides, estrogen receptor positive, A549, MCF-7

SUN Jie, WANG Shao, GUO Bin, WEI Ya-Hui. Flavonoids of Rhizoma Sparaganii Induce S/G₂ Stage Arrest in A549 and MCF-7 Cells[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 224-227.

References

1 Gruber CJ,Tschugguel W,Schneeberger C,et al.Production and actions of estrogens.New Engl J Med,2002, 346:340-352.
2 Yager JD,Davidson NE.Estrogen carcinogenesis in breast cancer.New Engl J Med,2006,354:270-282.
3 Mollerup S,Jorgensen K,Berge G,et al.Expression of eatrogen receptors α and β in human lung tissue and cell lines.Lung Cancer, 2002, 37:153-159.
4 Villa E,Colantoni A,Grottola A,et al.Variant estrogen receptors and their role in liver disease.Mol Cell Endocrinol,2002,193:65-69.
5 Andreetta C,Smith I.Adjuvant endocrine therapy for early breast cancer.Cancer Lett,2007,251:17-27.
6 Cheung KL.Endocrine therapy for breast cancer: an overview.The Breast,2007,16:327-343.
7 Barthelmes L,Gateley CA.Tamoxifen and pregnancy.The Breast,2004,13:446-451.
8 Kim IG,Kang SC,Kim KC,et al.Screening of estrogenic and antiestrogenic activities from medicinal plants. Environ Toxicol Pharmacol,2008,25:75-82.
9 Li A(李傲),Xu XY(徐晓玉),Wang H(王慧),et al.Study on inhibitory effect of medicated serum of SLW on estrogen production by human endometrial cells of endometriosis.China J Chin Mat Med(中国中药杂志), 2008,33:686-690.
10 Powell CE,Soto AM,Sonnenschein C.Identification and characterization of membrane estrogen receptor from MCF-7 estrogen-target cells.J Steroid Biochem,2001,77:97-108.
11 Zhang WD(张卫东),Wang YH(王永红),Qin LP(秦路平).Chemical constituents of flavoniods from Sparganium stoloniferum.China J Chin Mat Med (中国中药杂志),1996, 21:550-551.
12 Zhang TJ(张铁军),Wang LL(王丽莉),Study on the chemical constituents of Scirpus yagara.Modern Pharm Clinic (现代药物与临床),2009,24:36-38.
13 Shirota O,Sekita S,Satake M.Two phenylpropanoid glycosides from Sparganium stoloniferum.Phytochemistry, 1997,44:695-698.
14 Shirota O,Sekita S,Satake M.Chemical constituents of Chinese folk medicine “San Leng”,Sparganium stoloniferum.J Nat Prod,1996,59:242-245.
15 Laios I,Journe F,Laurent G,et al.Mechanisms governing the accumulation of estrogen receptor alpha in MCF-7 breast cancer cells treated with hydroxytamoxifen and related antiestrogens.J Steroid Biochem,2003, 87:207-221.
16 Paruthiyil S,Parmar H,Kerekatte V,et al.Estrogen receptor β inhibits human breast cancer cell proliferation and tumor formation by causing a G2 cell cycle arrest.Cancer Res,2004,64:423-428.

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Flavonoids of Rhizoma Sparaganii Induce S/G₂ Stage Arrest in A549 and MCF-7 Cells

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