NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (2): 275-278.

• Article • Previous Articles     Next Articles

Adsorption and Desorption of Alfalfa (Medicago sativa L.) Saponins by Macroporous Resin

WANG Ji-hua1 , ZHOU Yu-fan2, HE Wei2, HAN Jian-guo3, YU Zhu3, ZHOU Li-gang1*   

  1. 1College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; 2College of Forestry, Beijing Forestry University, Beijing 10083, China; 3College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
  • Received:2010-07-30 Online:2011-04-25 Published:2011-10-14
  • Supported by:

    This work was co-financed by the grants from the Commonweal Fund of China Agriculture (nyhyzx07-022), the National Key Technology R&D Program of China (2006BAD16B08), and the Introducing International Advanced Agricultural Project of the Ministry of Agriculture of China (2006-G38).

PDF (PC)

390

Abstract:

Five types of macroporous adsorption resins namely AB-8，S-8，NKA-9，D-101 and X-5 with varying polarity were evaluated for the adsorption and desorption of alfalfa saponins. Of these，the moderately polar AB-8 resin showed the highest adsorption capacity and the absorbed saponins could be eluted effectively with 55-65% aqueous ethanol. The adsorption reached saturation at 1:1 alfalfa extract to AB-8 resin mass ratio. The maximum desorption capacity of alfalfa extract was 108.4 mg/g dw AB-8 resin on a column eluted by 90% aqueous ethanol. The saponin content in the concentrated 90% aqueous ethanol effluent reached 53%，about 9 times of that (5.68%) in the crude alfalfa extract. The AB-8 macroporous resin has may be a favorable sorbent for large-scale saponin isolation from alfalfa.

Key words: Medicago sativa, saponins, macroporous resin, adsorption, desorption