NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (2): 299-303.

• Article • Previous Articles     Next Articles

Preparing Gallic Acid and Protocatechuic Acid in One Step from Polygonum capitatum Buch-Ham ex D. Don by High-speed Counter-current Chromatography

FANG Wei1 , WAN Jin-zhi1* , ZHANG Li-yan2, LIANG Bin3, CHEN Xin-xia   

  1. 1Laboratory for Pharmaceutical Analysis and Quality Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China; 2Department of Pharmaceutical sciences, Guiyang College of Traditional Chinese Medicine, Guiyan 550002, China; 3Warmen Pharmaceutical Co., Ltd., Guiyan 550018, China

  • Received:2010-03-25 Online:2011-04-25 Published:2011-10-14

Abstract:

A HPLC method was established for the determination of chemical components from polygonum capitatum Buch-Han ex D.Don. And high-speed counter-current chromatography was successfully applied to isolate and purify phenolic constituents from the crude extract of Polygonum capitatum. The solvent system used was hexane-ethyl acetate-methanol-water (1: 5: 1: 5,v/v/v/v),the rotate speed was 840 r/min,the flow rate was 2 mL/min,and the effluent was detected at 272 nm. 148.5 mg of gallic acid and 9.2 mg of protocatechuic acid were separated from 756.0 mg extract with the purities of 99.8% and 97.4%,respectively. The structures of the compounds were identified by UV,IR,LC/MS,1H and 13C-NMR.

Key words: Polygonum capitatum, HSCCC, HPLC, selection of solvent system, gallic acid, protocatechuic acid