Purification and Characterization of a Novel Metalloproteinase AA-MP-I from the Venom of Agkistrodon acutus

Abstract

Abstract:

A novel P-I snake venom metalloproteinase AA-MP-I was purified from the venom of Agkistrodon acutus by using thiophilic adsorption chromatography, Sephadex G-75, Affi-gel blue gel, and POROS HQ 20 anion-exchange chromatography. AA-MP-I was a monomer with the molecular weight of approximate 22.9 kDa on SDS-PAGE and its isoelectric point was 5.55 analyzed by IEF. AA-MP-I hasn’t any neutral carbohydrate and its N-terminal amino acid sequence was STEFQRYMEIVIVVDHSMVK. AA-MP-I has basic proteolytic and antithrombin activities, both of which are heat-unstable. In addition, AA-MP-I was proved to have hemorrhagic activity but no acidic phospholipase A₂ activity.

Key words: Agkistrodon acutus, venom, metalloproteinase, antithrombin, hemorrhage activity

PAN Jian-Ru, HE Huo-Cong, LIU Fang, RAO Ping-Fan. Purification and Characterization of a Novel Metalloproteinase AA-MP-I from the Venom of Agkistrodon acutus[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2010, 23(6): 929-933.

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