Chemical Composition and Antibacterial Activity of the Volatile Oil from the Endophytic Fungus Dzf12 Associated with Dioscorea zingiberensis

Abstract

Abstract:

The volatile oil was extracted with a yield of 0.276% on fresh weight basis by hydro-distillation from Dzf12, an endophytic fungus isolated from Dioscorea zingiberensis. The oil was analyzed for its chemical compositions by gas chromatography-mass spectrometry (GC-MS). Methyl hexadecanoate (22.63%), methyl (E)-9-octadecenoate (8.30%), methyl (E,E)-9,12-octadecadienoate (8.12%), 2,6-di-tert-butyl-4-methylphenol (6.79%), tetradecane (6.69%), and dodecane (5.88%) were the major compounds of the 30 identified components which accounted for 95.47% of the total volatile oil. The in vitro antibacterial activities of the oil on eight bacteria were investigated. The minimum inhibitory concentration (MIC) and median inhibitory concentration (IC₅₀) values of the oil against the test bacteria ranged from 0.75 mg/mL to 2.00 mg/mL and from 0.62 mg/mL to 1.55 mg/mL, respectively. One of the most sensitive bacteria was Xanthomonas vesicatoria with the MIC and IC50 values to be 0.75 mg/mL and 0.62 mg/mL, respectively. These results indicated that the endophytic fungus Dzf12 would be a candidate for producing biodiesel as well as a potential source for producing antimicrobial components.

Key words: Dioscorea zingiberensis, endophytic fungal isolate Dzf12, volatile oil, antibacterial activity

DAN Ti-Jiang, ZHAO Jiang-Lin, WANG Ji-Hua, ZHANG Ying, QIU Yi, SUN Huan, ZHOU Li-Gang. Chemical Composition and Antibacterial Activity of the Volatile Oil from the Endophytic Fungus Dzf12 Associated with Dioscorea zingiberensis[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2010, 23(6): 952-955.

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