NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2010, Vol. 23 ›› Issue (6): 965-971.

• Article • Previous Articles     Next Articles

Antioxidation and Immunomodulatory Function on Lymphocyte Subsets of Salvianolic Acid B in High-fat Diet Fed Mouse

WANG Bin2 , CUI Jue2 , XIAO Ying2 , SHI Yong-hui1,2 , LE Guo-wei1,2*   

  1. 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2Institute of Nutrition and Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

  • Received:2009-02-25 Online:2011-01-10 Published:2011-10-19

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Abstract:

This article illustrates the effects of Salvianolic acid B (Sal B) on the antioxidation and immunomodulatory function of lymphocyte subsets in high-fat diet fed mouse. Forty male C57BL/6 mice were randomly assigned to four groups. The control group consumed an ordinary diet. The other three experimental groups were fed with a high-fat diet, a high-fat diet plus 0.002% Sal B, and a high-fat diet plus 0.004% Sal B, respectively. After 8 weeks, the plasma lipid status, reactive oxygen species (ROS) level, actioxidase activity and the content of MDA in blood and spleen were examined. The proportion of lymphocyte subsets were determined by FACSAria Flow Cytometer. The results indicated that supplementation of Sal B could decrease the levels of plasma lipid and ROS and increase the antioxidation index of high-fat diet mice. It could increase the ratio of CD4+ and CD8+ T cell as well as the ratio of CD19+ cell in experimental groups. The antioxidation and immunoregulation effects of low dose of Sal B were better than those of the high dose.

Key words: high-fat diet, Salvianolic acid B, plasma lipid, reactive oxygen species, antioxidase, lymphocyte subsets