NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2010, Vol. 23 ›› Issue (6): 1053-1056.

• Article • Previous Articles     Next Articles

Isolation, Purification and Structural Analysis of PG-g from Panax ginseng

WANG Ying1，GAO Qi-pin2，LI Gui-rong3，CHEN Ying-hong4，JIANG Rui-zhi4*   

  1. 1College of Light Industry and Food, South China University of Technology, Guangzhou 510641, China; 2Center for New Medicine Research, Changchun University of Chinese Medicine, Changchun 130117, China; 3School of Pharmacy, Yanbian University, Yanji 133000, China; 4Academy of Chinese Medicine and Material Medica of Jilin Province, Changchun 130021, China

  • Received:2009-07-21 Online:2011-01-10 Published:2011-10-19

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Abstract:

In this paper, the aqueous extract of P. ginseng root was taken as experiment material. It was forced through Cellulose Super Filtration Systerm to obtain the ultrafiltrate. The ultrafiltrate was loaded onto a Carbon-Celite column and fractionated into five parts by eluting with water, 5%, 30%, 60%, and 95% ethanol. Chemical analysis of the five fractions showed that the carbohydrate content of 30% ethanol elution (PGO) was the highest. Then PG-g was obtained from PGO which was purified by using Sephadex G-15 and DEAE-Sephadex A-50 chromatography. The PG-g was composed of Ara, GalA, and GlcA. Methylation analysis showed that GlcA and GalA were the non-reducing termination, (1→3)-linked GalA and (1→6)-linked GlaA were the backbone, C3 of GlcA and C6 of GalA existed branch chain, and also existed (1→2,4)-linked Ara. Peptide was glycosidically linked through hydroxyprolinyl arabinosides. And PG-g contained 14 kinds of amino acid, of which Cys and Tyr were the the most abundant amino acid.

Key words: Panax ginseng, glycopeptide, structure, methylation