Abstract: In this paper,the secondary metabolites of anthraquinones were obtained by fermentation of endophytic fungi of Rumex gmelini Turcz..HPLC was used to filter different anthraquinones from endophytic fungi of R. gmelini.With 5 anthraquinone compounds,including emodin,rhein,chrysophanol,physcion and aloeemodin as reference,the endophytic fungi with the secondary metabolites of anthraquinones were screened out,in which 3 strains were screened out to contain emodin,3 strains contained rhein,and 5 strains contained aloe-emodin.Among these fungi,the highest and lowest yield of anthraquinones was 683 μg/g and 8 μg/g,respectively.The strains had higher concentrations of anthraquinones between each strains and its respective separated parts in R. gmelini. The developed HPLC method was accurate and fast,and it was applicable to analysis metabolites of endophytic fungi from R. gmelini.

Key words: Rumex gmelini Turcz., endophytic fungi, HPLC, anthraquinones secondary metabolites