Abstract: In this study,the concentrations of 7hydroxycoumarin,xanthotoxol,imperatorin and phellopterin in the tissue culture and cultivated samples of Changium smyrnioides were determined by HPLC.The Agilent C18 column (250 mm×4.6 mm,5 μm) was used for the chromatographic separation with a mobile phase of acetonitrile-water solution in gradient elution mode.The flow rate was 1.0 mL/min.The column oven temperature was controlled at 30 ℃.The detection wavelength was set at 314 nm and the injection volume was 10 μL.The experimental results showed that the concentrations of the 4 investigated coumarins of the cultivated sample in cultivated Changium root cortex was generally higher,which were 5.8970,1.2710,0.0301 mg/g and 0.9236 mg/g,respectively.The concentrations of 7hydroxycoumarin and xanthotoxol in callus induction of Changium root were 3.7939 mg/g and 0.8384 mg/g,respectively.The developed HPLC method was simple,accurate and reproducible.It was suitable for assaying the contents of coumarins in the tissue culture and the cultivated sample of C.smyrnioides.

Key words: Changium smyrnioides Wolff, tissue cultures, HPLC, coumarins, determination