NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (8): 1178-1181.

• Article • Previous Articles     Next Articles

Mutational Screening of Lyophyllum decastes with High Yield of  Laccase and Characteristics of Laccase from the Mutant

LIANG Qian-qian1,2, WEI Sheng-long1, DING Ling-qiang2, YAN Hong-xia2, LIANG Yan-ping2, XI Ya-li1*   

  1. 1 Institute of Edible Fungi,Hexi University; 2 Department of Life Science and Engineering,Hexi University,Zhangye 734000 ,China
  • Online:2014-08-30 Published:2014-11-06

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Abstract: The protoplasts of one Lyophyllum decastes were exposed to UV radiation and the mutants were screened on guaiacol-containing PDA plates.The laccase activities of the mutated strains were further determined by ABTS.A mutant strain HY 1022-01 with high production yield of laccase was obtained.The further investigation on the characteristics of laccase from mutant strain HY 1022-01 showed that the maximum laccase activity increased 30.32% compared to its parent strain and up to 991.67 U/mL in optimum condition.The laccase activity was stable in 30-35 ℃ and pH of 2.2-3.8,while the optimal temperature and pH was 35 ℃ and 3.0 respectively.The Km was 0.72622 μM on the basis of ABTS.

Key words: Lyophyllum decastes, laccase, ultraviolet mutagenesis

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