Abstract: Glycyrrhetinic acid-cisplatin complex (GA-Pt) was prepared by the combination of natural compound 18β-glycyrrhetinic acid and anticancer drug cis-Platin within a three-step reaction.The in vitro anticancer activity of the synthesized complex was studied using human Bel-7402 hepatocarcinoma cell lines.Cell viability was determined by MTT method.Statistical analysis showed that GA-Pt inhibited Bel-7402 cell proliferation significantly.The inhibitory rate was 29.31%-92.25% at the concentration range of 5-400 μg/mL.The experimental results suggested that the human Bel-7402 hepatocarcinoma cells have a relatively high sensitivity to GA-Pt.The combination of glycyrrhetinic acid with cis-Platin increased their anticancer activity.This may be related to the synergistic anti-cancer property and chemotherapy sensitizing effect of GA to Cis-platin.This work provided an initial basis for pharmacological screening of new liver-targeting anticancer agents from glycyrrhetinic acid-cisplatin complex.

Key words: Glycyrrhetinic acid-cisplatin complex, preparation, anticancer activity