Abstract: This study is to establish the high-speed counter-current chromatography method for purification of effusol,dehydroeffusol,juncusol,dehydrojuncusol from Juncus effusus L. A two-phase solvent system was composed of cyclohexane-ethyl acetate-methanol-water(5:3:5:3),The upper phase was stationary phase while the lower as mobile phase,the flow rate was 2 mL/min,the revolution speed was 850rpm,and the emperature was 25 ℃.The ethyl acetate extracts of Juncus effusus was purified by HSCCC twice.The purity of effusol,dehydroeffusol,juncusol,dehydrojuncusol was 99.3%,98.5%,98.5%,97.4%.

Key words: Juncus effusus, phenanthrenes, High-speed counter-current chromatography, separation