NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (8): 1257-1260.

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Determination of Total Dihydroflavonols in Extract of Engelhardtia roxburghianan Wall. Leaves

FU Xiao-xue1, SONG Ming-ming1, ZHOU Hui2, SHI Li-ying1, TANG Ling1, WANG Yong-qi1*   

  1. 1 Institute of Materia Medica of Dalian University,Dalian 116622 ,China;2 Agricultural Machinery Technology College of Qinzhou,Qinzhou 535000 ,China
  • Online:2014-08-30 Published:2014-11-06

Abstract: The objective of this study was to determine the content of total dihydroflavonols in different extracts of Engelhardtia roxburghiana wall.leaves.The contents of total dihydroflavonols in ethanol extract and its PE,EtoAc and water fractions as well as water extract and its EtoAc and water fractions of E.roxburghiana leaves were determined by spectrophotometry with astilbin as standard.The results indicated that astilbin showed a good linear relationship in the range of 12.3~28.7μg/mL (R2=0.9991); the developed method was with high sensitivity,good stability,and it is applicable for the quality control of E.roxburghiana leaves.

Key words: Engelhardtia roxburghiana Wall.leaves, total dihydroflavonols, astilbin

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