NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (8): 1281-1284.

• Article • Previous Articles     Next Articles

Reversal of Multidrug Resistance in SGC7901/ADR Cells by Rabdosia rubescens

GAO Qiao-hui, XU Wen-ting, XIE Ting, LIU Rui-yuan, WU Zhen, LI Fa-rong*   

  1. Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry,Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China,College of Life Sciences,Shaanxi Normal University,Shaanxi Xi’an 710062 ,China
  • Online:2014-08-30 Published:2014-11-06

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Abstract: The effect of Rabdosia rubescens on the expression and function of P-glycoprotein (P-gp) in SGC7901/ADR cell was investigated in this study. The potential bioactive extract fractions were selected to carry out experiments in SGC7901/ADR cells. After treatment with chloroform fraction and ethyl acetate fraction of R. rubescens, the cell viability was examined by MTT assay,the expression of P-gp in SGC7901/ADR and SGC7901 cells was determined by fluorescent-labeled antibody technique,and the expression of mdr1 gene was detected by RT-PCR. The experimental results indicated that chloroform fraction and ethyl acetate fraction of R. rubescens increased the intracellular accumulation of adriamycin in SGC7901/ADR cells,and decreased the expression of mdr1 gene and P-gp. Therefore, chloroform fraction and ethyl acetate fraction of R. rubescens had effects on reversal of multidrug resistance of SGC7901/ADR cells,and the mechanism was possibly associated with the inhibition of the expression of P-gp.

Key words: Rabdosia rubescens, SGC7901/ADR, multidrug resistance, P-glycoprotein

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