Abstract: The objective of this study was to investigate the antioxidant activities of by-products of Ganoderma lucidum spores.Ethanol soluble extract of by-products of Ganoderma lucidum spores was prepared by 80% alcohol,followed by fractional extraction respectively with petroleum ether,chloroform,ethyl acetate and n-butanol.Then the antioxidant activities of above five different fractions were evaluated by T-AOC and free radical scavenging power like DPPH· and ·OH to identify the most efficient fraction.The results indicated that all fractions showed high radical scavenging efficiency,especially the ethyl acetate fraction (EA) which was chosen to take a further study on anti-lipid peroxidation.In this study,the protective effects of EA at 0.5 mg/mL on β-carotene/linoleic acid system was 70.32%.The inhibitory activity of EA at 2 mg/mL on hemolysis of erythrocytes and lipid peroxidation of mice homogenate induced by H₂O₂ were 41.39% and 51.00%.Taking together,each extract has significant antioxidant activities,especially the ethyl acetate extract.

Key words: by-products of Ganoderma lucidum spores, free radicals, ethyl acetate extract, lipid peroxidation