Abstract: In this study,MNNG and other comprehensive factors were used to establish gastric precancerous lesions (GPL) model.80 mesh,100 mesh,80 mesh and 100 mesh mixture of compound lizards powder,normal saline and vitacoenzyme were applied on the GPL model,respectively.The protein expression of Wnt2, β-catenin and Cyclin-D1 was detected using immunohistochemical method.The pathological and histological changes of gastric mucosa were observed with optical microscope,and the optical density of immunohistochemical image was calculated through the image analysis system.The experimental results showed that:the positive expression rate of cell proliferation gene Wnt2,β-catenin and Cyclin-D1 protein in each group treated with the compound lizards powder was significantly lower than that of the model group (P<0.01).The positive expression rates of the 3 proteins in the 80 mesh and 100 mesh compound lizards powder mixture group was significantly lower than those of vitacoenzyme group (P<0.05).This revealed that the complex lizards powder can repair the damage of gastric mucosa and treat or reverse the development of PLGC,of which the mechanism might be that the excessive proliferation of Wnt2,β-catenin and Cyclin-D1 was inhibited through the intervention of the proliferation and differentiation of the mucosa to promote cell apoptosis and restore the balance of cell proliferation and apoptosis,thus block the precancerous lesions of stomach cancer.

Key words: gastric precancerous lesions, the compound lizards powder, Wnt signal pathway, Wnt2, beta-catenin, Cyclin-D1, proliferation