Abstract: Nine steroidal compounds were isolated from the rhizomes of Tupistra chinensis, which were respectively identified as 3-epi-neoruscogenin-3-β-D-glucopyranoside (1), 3-epi-ruscogenin-3-β-D-glucopyranoside (2), ranmogenin A (3), convallagenin B (4), 3-epi-neoruscogenin ( 5), tupichigenin E (6 ), (20S,22R)-spirost-25(27)-ene-1β,2β,3β,4β,5β,7α-hexaol-6-one ( 7 ), β-sitosterol (8) and daucosterol (9) by analyzing their spectral data and comparing with the previously reported literatures.Compounds 1,2 and 4 were isolated from this plant for the first time.

Key words: Tupistra chinensis, steroids, structural identification