Abstract: Fourteen compounds were isolated from the leaves of Eucommia ulmoides Oilv.by several methods,including silica gel,reversed phase RP-18 column chromatographies,Sephadex LH-20,and HPLC.Their structures were identified by NMR and EI-MS spectra,as kaempferol (1),quercetin (2),hirsutin (3),astragalin (4),(+)-1-hydroxylpinoresinol (5), (+)-1-hydroxy-6-epipinoresinol (6), pinoresinol-4-O-glucoside (7), thunberginol C (8), (+)-medioresinol-4′-O-β-D-glucopyranoside (9), 7-hydroxycoumarin (10),methylchlorogenate (11),leinoleic acid (12),butanedioic acid (13), β-sitosterol (14).Compounds 6,8,10,12 and 13 were isolated from E.ulmoides for the first time.

Key words: Eucommia ulmoides Leaves, chemical constituents, structure elucidation, flavones