Abstract: Simultaneous determination of 3 stilbene compounds,namely resveratrol,polydatin and stilbene glucoside (2,3,5,4′-tetra-hydroxy-stilbene-2-O-β-D-glucoside) from Arachis duranensis was performed using HPLC-DAD and UPLC-MS/MS.The experimental conditions for HPLC-DAD analysis were as follows:ODS1 column (250 mm × 4.6 mm,5 μm);column temperature,40 ℃;mobile phase A,methanol;mobile phase B,1% aqueous acetic acid;flow rate,1.0 mL/min;DAD scanning wavelength,190-400 nm;detection wavelength,303 nm.The UPLC-MS/MS conditions were as follows:ACQUITY UPLC BEH C₁₈ column (2.1 mm × 50 mm,1.7 μm);mobile phase A,acetonitrile;mobile phase B,water;ion source,ESI (negative ion mode).UPLC-MS/MS data were collected using multiple reaction monitoring (MRM) mode.The results showed that resveratrol contents were 66.28,90.83 and 81.56 μg/g,polydatin contents were 123.78,302.77 and 236.53 μg/g,in roots,stems and leaves of A.duranensis,respectively.The content stilbene glucoside in roots and stems was 673.60 and 764.65 μg/g,respectively,but was not detected in leaves.

Key words: Arachis duranensis, stilbene compounds, HPLC-DAD, UPLC-MS/MS