NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (5): 709-712.

• Article • Previous Articles     Next Articles

Isolation of Components with Anti-angiogenesis Activity from Albizia julibrissin by Solid Phase Extraction Coupled with Preparative HPLC

CAI Wei-wei1, SHI Jian-jun1, FENG Lei2, DU Bin2, QIU Li-ying1,2*   

  1. 1 School of Pharmaceutical Science,Jiangnan University; 2 Wuxi Medical School,Jiangnan University,Wuxi 214122,China
  • Online:2014-05-31 Published:2014-11-17

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Abstract: In this study,several components with anti-angiogenesis activity were obtained from the n-butanol fraction of ethanol extract of Albizia julibrissin by solid phase extraction (SPE) coupled with preparative HPLC.The anti-angiogenesis effect and the composition analysis of the isolated components were monitored on HMEC-1 cells and by HPLC method,respectively.Finally,the component II-4 with higher anti-angiogenesis activity on HMEC-1 cells was obtained (IC50=1.45±0.11 μg/mL), and its composition was identified.The SPE-HPLC method was simple and fast,and can be used for rapid separation of Chinese herbal medicine at the same time it provided a reliable experimental materials for further separation of monomer compounds.

Key words: Albizia julibrissin, solid phase extraction, HPLC, anti-angiogenesis

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