Abstract: Chickpea seeds were widely used as raw material to extract ferritin.To maximize the extraction yield of ferritin,extraction parameters,including buffer types,buffer pH,solidliquid ratio,type and concentration of salt were investigated by single factor experim-ents followed by four factorthree level orthogonal experiments.The extraction yield of ferritin,total protein and dry weight in chickpea seeds were examined.The results showed that the effect of the investigated factors on the extraction yield of chickpea ferritin was in sequence of solidliquid ratio > salt concentration > temperature > pH.The optimal extraction condition was as follows:solid-liquid ratio 1:4, 70 mmol/L MgCl₂,extraction temperature 50 ^oC, KH₂PO₄-NaOH buffer solution at pH 7.5.The optimal extraction yield was 0.002643%.

Key words: chickpea, ferritin, extraction technology, orthogonal text