Abstract: The objective of this study was to study the pharmacokinetics of oleuropein extracted form Jasminum officinale L.var.grandiflorum in healthy rats.Male Wistar rats were randomly divided into 100,200,400 mg/kg of oleuropein group,The rats were intragastric administrated (i.g.) at different time.After the drug administration, blood sampling, centrifugal, plasma protein precipitation with methanol and acetonitrile were carried out.The concentration of oleuropein in blood serum was determined by high performance liquid chromatography (HPLC) at different time points.The average blood oleuropein concentrationtime data set was used to analyze the pharmacokinetic parameters by 3P97 software.The pharmacokinetic parameters between different groups were applied to SPSS 17.0 software for statistical analysis.The results showed that the pharmacokinetics of oleuropein in rats was fitted with two compartment model.The t_1/2,CL/F,AUC of low,medium and high dose sample group had significant difference.The t_1/2 and CL/F increased with the increasing of dose,while the AUC decreased firstly and then increased with increasing of dose.These results proved that the pharmacokinetics of oleuropein in rats were a non-linear process.

Key words: oleuropein, HPLC, pharmacokinetics