Abstract: Two pimarane diterpenes were isolated from the fermentation broth of the Arctic-derived fungus Eutypella sp.D-1 by various chromatographic methods.On the basis of ¹H, ¹³C and 2D-NMR spectral data,compounds 1 and 2 were identified as libertellenone C (1) and libertellenone A (2).The potential anticancer activity and antifungal activity against conidia of Pyricularia oryzae of compounds 1 and 2 were evaluated.Compound 1 showed weak cytotoxicities against two tumor cell lines SW-1990 and U-251,compound 2 exhibited antifungal activity against conidia of Pyricularia oryzae with the MIC of 18.8 μg/mL.

Key words: Arctic-derived fungus, Eutypella sp., secondary metabolites, pimarane diterpenes