Analysis on Chemical Components from Water Extract of Angelicae pubescentis Radix by High Performance Liquid Chromatography-electrospray-Ionizationquadrupole-time of Flight-mass Spectrometry

NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (1): 69-76.

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Analysis on Chemical Components from Water Extract of Angelicae pubescentis Radix by High Performance Liquid Chromatography-electrospray-Ionizationquadrupole-time of Flight-mass Spectrometry

SUN Dong-dong1, XU Xiao-fang2, YAN Shi-hai3, SONG Xiao-mei2, LI Xiang1*

1 College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China;2 School of Pharmacy, Shaanxi University of Chinese Medicine,Xian Yang 712046,China; 3 Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China

Online:2014-01-31 Published:2014-12-15

Abstract

Abstract: The manuscript make a qualitative analysis on chemical components in water extract of Angelicae Pubescentis Radix by HPLC-ESI-Q/TOF-MS;LC-MS/MS,C₁₈(4.6mm×250 mm,5μm)column was adopted,with methanol-0.1%formic acid as the mobile phase for gradient elution;ESI ion source was used for mass spectra, and data were collected in positive and negative mode.twelve compounds from water extract of Angelicae Pubescentis Radix were identified by analyzing positive and negative ion mass spectra information and element composition and comparing controls with data from relevant literature,these provide powerful therapeutic material basis for water extract of Angelicae Pubescentis Radix.

Key words: water extract of Angelicae pubescentis Radix, chemical components, HPLC-ESI-Q/TOF-MS

CLC Number:

R284.1

SUN Dong-dong1, XU Xiao-fang2, YAN Shi-hai3, SONG Xiao-mei2, LI Xiang1*. Analysis on Chemical Components from Water Extract of Angelicae pubescentis Radix by High Performance Liquid Chromatography-electrospray-Ionizationquadrupole-time of Flight-mass Spectrometry[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(1): 69-76.

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Analysis on Chemical Components from Water Extract of Angelicae pubescentis Radix by High Performance Liquid Chromatography-electrospray-Ionizationquadrupole-time of Flight-mass Spectrometry

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