Abstract: A comprehensive identification method for distinguishing Polygonum cuspidatum and grape vine source resveratrol was established based on UPLC-Q/TOF MS technology.The UPLC analysis of the resveratrol extracts from P.cuspidatum and grape vine were performed on Xunion C₁₈ column (4.6 mm × 150 mm,2.8 μm) using acetonitrile-water as mobile phases with the flow rate of 0.2 mL/min and detected at 287 nm in room temperature.Fingerprints of the resveratrol extracts were established based on eight resveratrol samples of P.cuspidatum source and grape vine source.Results showed that there were 13 common peaks on UPLC-UVfingerprint for P.cuspidatum extract and 21 common peaks for grape vine extract.In addition,11 peaks were identified according to MS information,and 4 of them were further confirmed by reference substance.In the present study,the 4 identified peaks and 3 characteristic fingerprint sections were found to be able to effectively distinguish the resveratrol extract from P.cuspidatum and grape vine source.It was believed that the identification method was of high precession and good stability.It provided some valuable insights into the identification and quality evaluation of resveratrol source.

Key words: Resveratrol, finger print, UPLC, Q/TOF