Abstract: Fifteen compounds were isolated from Hypericum ascyron L.by repeated column chromatography on silica,Sephadex LH-20 gel,and HPLC.On the basis of MS and NMR spectra,they were identified as 9,9′-O-di-(E)-feruloyl-(-)-secoisolariciresinol (1),kaempferol (2),quercetin (3),hyperoside (4),quercetin-3-O-β-D-glucopyranoside(5), kaempferol-3-O-β-D-galactopyranoside(6),kaempferol-3-O-β-D-glucopyranoside (7),protocatechuic acid (8),1,7-dihydroxyxanthone (9),trans-p-coumaric acid (10),p-hydroxybenzoic acid (11),kaempferol-3-O-(6”-O-crotonoyl)-β-D-glucopyranoside (12),1,3,6,7-tetrahydroxyxanthone (13),gallic acid (14) and β-sitosterol (15) respectively.Among them,compounds 1,4 and 6-14 were isolated from the species for the first time.

Key words: Hypericum ascyron L., chemical constituent, flavonoid