Abstract: The anti-UV efficacy and safety of extracts of Cannabis sativa L.(China-hemp) of different growth stages and/or different parts were evaluated by means of ultraviolet spectral scanning,free radical scavenging tests and light hemolysis experiments.The screened out extract with the highest anti-UV efficacy was the 95% ethanol extract of hemp leaves (160 days).The optimized extraction parameters were determined as follows:solidliquid ratio 1:20,extraction temperature 75 ℃,extraction time 2 h,and extract for one time.The main component of the extract was flavonoids (0.571 g/g).In the safety evaluation,the results of red blood cell hemolysis test and chorioallantioc membrane experiment showed that the hemp leaf extract was not irritative.In addition,the hemp extract also had a good stability in solution with changing pH,cold,heat and light.The 95% ethanol extract of hemp leaves (160 days) had the potential to be used as a functional plant additive for anti-UV cosmetics.

Key words: China-hemp, ultraviolet, free radical-scavenging, light hemolysis test, safety evaluation