Abstract: A high performance liquid chromatography (HPLC) method was developed and validated to determine the contents of scopoletin in the wild and cultivated FructusLycii from Qaidam Basin.The component was separated on aDiamonsil C₁₈ columnusing methanol-0.05%phosphoric acid buffer (35:65) as the mobile phases.The flow rate was 1 mL/min.The column temperature was set at 30 ℃and the detection wavelength was set at 344 nm.It was found that the linearity range of scopoletin was from 0.026-1.040 μg (R²=0.9996).The average recoveries (n=6) were 96.1%,RSD=2.0%.The method was accurate,reliable and reproduciblefor the determination ofscopoletin in FructusLycii.The result showed that the quality of cultivated FructusLyciifrom Qaidam Basin was neatly excellent.However,the wild samples had the significant different qualities,which was suitable for the selective exploitation.

Key words: HPLC, scopoletin, Qaidam Basin, FructusLycii