Abstract: Simultaneous extraction and purification technology of tanshinone and salvianolic acid was investigated in this work.With the contents of tanshinoneⅡA and salvianolic acid B as evaluation indexes,the effects of ethanol concentration,ethanol volume and percolation rate on extraction of tanshinoneⅡA and salvianolic acid B were investigated by orthogonal test.Types of macroporous resin,pH of sample solution,volume of eluent and ethanol concentration were observed by single factor experiments.The optimized extraction process were as follows:sample of Salvia miltiorrhiza Bge.was crushed and filtered through 10 mesh sieve, percolated with 80% ethanol at 4 mL/(min·kg),collected 10 times amount of percolating liquid,recovered ethanol,diluted the solution to 0.5 g/mL of crude drug with water,adjusted pH to 3.0 by HCl,filtrated and got tanshinone.The sample solution was purified with HPD100 macroporous adsorption resin,washed with 2 BV of water,then eluted with 3 BV of 50% ethanol.The eluent was collected and got salvianolic acid.Under the optimized extraction and purification processes,good separation and high contents of tanshinone IIA and salianolic acid B was achieved.The developed process was simple,stable and reliable.It can be applied in large scale production.

Key words: extraction technology, separation and purification technology, tanshinoneⅡA, salvianolic acid B