Abstract: To study the chemical constituents of deep-sea fungus Penicillium sp.F00120,nine compounds were isolated from its EtOAc extract using silica gel,Sephadex LH-20,ODS and HPLC methods.Based on the spectroscopic analysis,their structures were identified as ergosterol (1),4-methyl-5,6-dihydro-2-pyranone (2), citreohybridonol (3),1-Linoleoylglycerol (4),5α,6α-Epoxy-(22E,24R)-ergosta-8(14),22-diene-3β,7β-diol (5),macrophorin (6),purpurogemutantin (7), 3-(2S,4S-dihydroxypentyl)-6,8-dihydroxyisocoumarin (8), 5-hydroxy-7-(2′-hydroxypropyl)-2-methyl-chromone (9).Compounds 2-4, 6-9 were isolated from Penicillium sp.F00120 for the first time.

Key words: deep-sea fungus, Penicillium sp.F00120, spectroscopic analysis, secondary metabolites