Isolation and Identification of Antibacterial Lipopeptide Produced by Bacillus amyloliquefaciens C-1

doi:10.16333/j.1001-6880.2015.02.001

Abstract

Abstract: Bacillus amyloliquefaciens C-1 is a newly isolated strain from ready-to-eat food.Its fermentation supernatant showed strong antibacterial activity.The crude lipopeptide was isolated by acid precipition method from C-1 fermentation supernatant.The antibacterial activity of this lipopeptide was verified.Then, Bacillus cereus MS10362R, Bacillus cereus CMCC63301 and Escherichia coli O157:H7 CDC157 were selected as the indicating bacterium to track the antibacterial activity.The results showed the inhibition rate of 0.1 mg/mL lipopeptide against those 3 strains were 96%,75% and 93%,respectively.In addition,C-1 lipopeptide had very stable antibacterial activity under high temperature,different proteases and solvents treatment.The results suggested that strain C-1 had the potential application value to be developed as new bio-preservative.

Key words: Bacillus amyloliquefaciens C-1, antibacterial activity, lipopeptide, food-borne pathogens

CLC Number:

Q939.9

LV Jia1,2, XU Xing-ke1,2, HU Sen-ke1,2, ZHANG Rui-juan1,2, HAN Bei1,2*. Isolation and Identification of Antibacterial Lipopeptide Produced by Bacillus amyloliquefaciens C-1[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(2): 199-204.

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Isolation and Identification of Antibacterial Lipopeptide Produced by Bacillus amyloliquefaciens C-1

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