Abstract: The effects of three macroporous adsorption resins D3520,DS-8 and DM-301 on the production of palmarumycins C₁₂ and C₁₃ in liquid culture of endophytic fungus Berkleasmium sp.Dzf12 were investigated in this study.All the tested resins enhanced the production of palmarumycin C₁₂.Only resin DM-301 enhanced the production of both palmarumycins C₁₂ and C₁₃.81.3% to 95.8% of palmarumycin C₁₂ was distributed in the resins.When resin DM-301 was applied to the medium at 4.17% (g/mL) on day 9,and then harvested after 112 h incubation,the maximal yields of palmarumycins C₁₂ and C₁₃ were obtained as 122.72 mg/L and 55.99 mg/L,which were 56.04-fold and 1.88-fold in comparison with those (2.19 mg/L and 29.86 mg/L) of the control,respectively.It can be an effective strategy for producing palmarumycins C₁₂ and C₁₃  in future large-scale culture of Berkleasmium sp.Dzf12 by adding macroporous resins.

Key words: endophytic fungus, Berkleasmium sp.Dzf12, spirobisnaphthalene, palmarumycins C₁₂ and C₁₃, macroporous resins, metabolic regulation