NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (2): 210-213.doi: 10.16333/j.1001-6880.2015.02.003

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Study on the Antioxidant Activity of Maojian Tea Polysaccharides and Its Conjugate

QU Wei1, WANG Meng-jun1, YU Hai-yan3, LIU Yu3, TIAN Xu-dong3, FAN Hai-tao2,3,4*   

  1. 1 Beijing Union University,Beijing 100101,China;2 Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China; 3 College of Bioengineering,Beijing Polytechnic,Beijing 100029,China; 4 Pharmaceutical College,Peking University Health Science Center,Beijing 100191,China
  • Online:2015-02-15 Published:2015-02-15

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Abstract: The antioxidant activity and free radical clearing function of Maojian tea polysaccharides and its conjugate were investigated.The polysaccharides (MP) were extracted and purified from Maojian Tea by common methods including Sevag and acetone treatment.MP was hydrolyzed completely in TFA and the hydrolysates were separated into two parts (MP-HCM and MP-HCW) by C18 chromatographic treatment.The antioxidant activity and free radical clearing function of MP,MP-HCM and MP-HCW were studied by DPPH method and Prussian Blue method.The results showed that the DPPH radical scavenging rates of MP,MP-HCM and MP-HCW were 28.99%,13.1% and 23.2%,respectively.The deoxidization indexes of were 0.044,0.015 and 0.029,respectively.The presented results confirmed both Maojian tea polysaccharides and its conjugates had capacity of eliminating DPPH radicals and had antioxidant activity.

Key words: Maojian tea, polysaccharides, conjugate, activity contribution

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