Abstract: In this study,UPLC-TOF-MS was used to compare the contents of gingkolides and bibobalide in the leaves of Ginkgo biloba L.from different species in the southwest of Hubei province.The separation was performed on a ZORBAX C₁₈ (2.1×50 mm,1.8 μm) column with acetonitrile and 0.1% formic acid in water as mobile phases at a flow rate of 0.2 mL/min,and the column oven temperature of 25 ℃.TOF mass spectrometry was used as the detector,and EIC mode was selected.The calibration curve was linear in the range of 0.2-4 μg/mL,with R² being larger than 0.9996 and RSD being smaller than 1.65%.The developed method was rapid,selective,accurate and suitable for the quantitative analysis of gingkolides and bibobalide.Among the different species,the maximum contents of gingkolides and bibobalide were found in the Eng23 species.Ratio of gingkolides verus bibobalide in the leaves of EnG23 were better than others.

Key words: UPLC-MS, gingkolide, bibobalide