NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (2): 294-300. doi: 10.16333/j.1001-6880.2015.02.021

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Extraction and Deproteinization of Polysaccharides from Guizhou wild Paederia scandens

CHANG Fei1,2*, WANG Shao-yun1, CHEN Fei1   

  1. 1 College of Chemistry and Materials Engineering,Kaili University,Guizhou Kaili 556011,China; 2 Center for Research and Development of Fine Chemicals,Guizhou University,Guizhou Guiyang 550025,China
  • Online:2015-02-15 Published:2015-02-15

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Abstract: A process for extraction of polysaccharides from Guizhou wild Paederia scandens powder was optimized through single factor test and orthogonal test.Four different methods,namely Sevag,TCA,Sevag-TCA,and HClO4 methods,for the removal of protein were investigated.The results showed that HClO4 method was demonstrated to be the best way for the removal of protein,and a protein emergence rate of 94.78% can be achieved after deproteinizing protein for 3 times.Moreover,15.14% polysaccharide loss rate was detected under the optimal extraction conditions of using water as solvent,solid-liquid ratio of 1:50,and ultrasonic power of 70 W at a bath temperature of 70 ℃ within an extraction time of 110 min repeating for two times.This process was simple and low cost.

Key words: Paederia scandens (Lour.) Meer., polysaccharide, extraction, deproteinization

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