NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (3): 466-469. doi: 10.16333/j.1001-6880.2015.02.020

• Article • Previous Articles     Next Articles

Determination of Total Flavonoids in Four Meconopsis Species from Qinghai-Tibet Plateau

SHE Li-ping1,2,SUN Jing1*,ZHOU Yu-bi1,SUN Sheng-nan1,2,ZHAO Qing-shuai1,2,YE Run-rong1, LU Xue-feng1,PENG Min1   

  1. 1 Qinghai Key Laboratory of QinghaiTibet Plateau Biological Resources,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China; 2 University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2015-03-30 Published:2015-04-07

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Abstract: In this paper,the contents of total flavonoids in four Meconopsis species collected from the Qinghai-Tibet plateau were determined by ultraviolet-visible spectrophotometer with colorimetric method.This method showed good linearity (r2=0.9990).The average recovery of the method was 101.56%;the accuracy and repeatability of the method were confirmed by the precision and repetition tests with RSD=2.94% and 1.34%,respectively.The results showed that the total flavonoids content in Meconopsis quintuplinervia was the highest,which was a top-grade medicinal herb in accord with the traditional usage habit of Tibet.In addition,the results showed that there was a remarkable difference between Meconopsis quintuplinervia and the others which was analyzed by SPSS 20.0 with ANOVA method.The results presented in this study,will provide a theoretical basis for the further studies of the medicinal herbs of Meconopsis species.

Key words: total flavonoids;colorimetric method;Meconopsis quintuplinervia, Meconopsis integrifolia, Meconopsis horridula, Meconopsis punicea

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