Abstract: The aim of this study was to investigate the subacute toxicity of polyprenols from G.biloaba L.(GBP).80 rats (female and male,each half) were randomly divided into 4 different groups,including GBP 417,833 mg/kg,and 1250 mg/kg (equivalent to 50,100 and 150 times of the recommended dose) groups and negative control group.When rats were continuously feeding in 30 days with GBP mixed into basal diet,routine test and biochemical indexes of blood were determined.The quantity of liver,kidney,spleen and testis were weighed,and histopathological examination were performed on liver,kidney,spleen,stomach,ovary and testis.During the period of 30 d’s feeding，the results showed that all rats performed normal at the end of the experiment,and the difference between different groups on the body weight,increasing weight,food intake,food utilization rate and organ/body weight ratio of rats were not statistically significant (P>0.05).The difference of routine indexes of RBC,WBC,HGB,NE,LY,MO,EO,BA as well as biochemical indexes of alanine aminotransferase,aspartate aminotransferase,urea,creatinine,blood glucose,total cholesterol,triglyceride,albumin and total protein,were also not statistically significant (P>0.05).Histopathological examination showed no specific lesions for organs.No apparent toxicity and no adverse effects to rats were observed by feeding 1250 mg/kg of GBPfor continuous 30 d.Therefore,GBP can be considered safe and nontoxic.

Key words: polyprenols from G.biloaba L., subacute toxicity, rat