NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (6): 1028-1031. doi: 10.16333/j.1001-6880.2015.06.016

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Antioxidant Activity of Fermentation Products of Entophytic -Fungal PFT-2 in Solanum cathayanum

YANG Yang 1,SU Xiang-ping1*, LI Ze-jun1,WANG Jun-zhi1,2, ZOU Kun1,2   

  1. 1 College of biological and pharmaceutical science,Three Gorges University;2Hubei Key  Laboratory of Natural Products Research and Development,China Three Gorges University,Yichang 443002,China
  • Online:2015-06-30 Published:2015-07-10

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Abstract: DPPH· scavenging assay and ferric reducing power assay in vitro were used to evaluate the antioxidant activities of the extracts from entophytic fungal PFT-2 in Solanum cathayanum. Firstly,the antioxidant activities of ethyl acetate extract from fermentation liquid and the methanol extract of mycelium were determined. Secondly,the antioxidant activities of petroleum ether fraction,chloroform fraction,ethyl acetate fraction, n-butanol fraction,acetone fraction,methanol fraction,water surplus parts from the total methanol extract of mycelium were tested,respectively.Simultaneously VC was used as positive control.The experimental results showed that all fractions of the methanol extraction of mycelium from entophytic fungal PFT-2 in S.cathayanum had strong scavenging capacity against DPPH·,in which ethanol fraction,acetone fraction and n-butanol fraction were almost equivalent to VC for DPPH· scavenging activity.The IC50 value of ethanol fraction for the DPPH· assay was 0.023 mg/mL,which suggested that antioxidant activity of ethanol fraction was even stronger than that of VC.Meanwhile,the above fractions all revealed strong Fe3+ reducing power.

Key words: Solanum cathayanum, entophytic fungal, fermentation, antioxidant activity

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