Abstract: In this paper,strains with higher cellulose degrading ability were isolated from cow manure by only carboxymethyl cellulose-Na (CMC-Na) medium.The morphological,physiological and biochemical characteristics and 16S rDNA gene were analyzed to identify the taxonomic position of the strains.Moreover,their cellulase producing conditions were also studied.The results showed that a strain (NF38) that can degrade cellulose effectively was obtained.According to taxonomic identification,the strain NF38 was identified as Streptomyces violascens. When its fermentation temperature ranged from 30 to 40 ℃ (optimum at 35 ℃) and pH 7.0 to 8.0 (optimum at 7.0),and inoculation amount at 5%-10% (optimum at 5%),it had the highest enzyme activity.In addition,it took 7d to reach the peak of cellulase production.In conclusion,strain NF38 had potential in degrading animal manure.

Key words: cow manure, cellulose decomposing microorganisms, identification, Streptomyces violascens