Abstract: This experiment was aimed to establish a RP-HPLC method for determining the contents of jasminoidin and schisandrae in Yinao Jieyu Granules.The experiment was carried out on an Eclipse XDB-C₁₈ column (250 mm×4.6 mm,5 μm) with gradient elution using methanol-water as mobile phases.The flow rate was 1.0 mL/min and the column oven temperature was set at 25 ℃.The detection wavelength was 238 nm (0-20 min) and 250 nm (20-40 min).The experimental result indicated that the linear range of jasminoidin was 0.2864-2.8640 μg (r = 0.9997),the average recovery was 98.82% and RSD was 1.53% (n=6);The linear range of schisandrae was 0.1062-1.0620 μg (r = 0.9999),the average recovery was 99.23% and RSD was 2.29% (n=6).The average contents of jasminoidin and schisandrae in 3 batches of samples were 8.53 mg/g and 2.82mg/g,respectively.The developed RP-HPLC method was simple,accurate,specific and reproducible. It can be used to control the quality of Yinao Jieyu Granules effectively.

Key words: HPLC, Yinao Jieyu Granules, jasminoidin, schisandrae