Abstract: To study the chemical constituents of entophytes (Myrothecium verrucaria) broth with agrochemical activities in Notopterygium incisum Ting ex H.T.Chang,fourteen compounds were isolated from its ethyl acetate extracts by silica gel,preparative HPLC and other column chromatography.On the basis of spectra data,they were identified as verrucarin B (1),8-acetoxyroridin H (2),verrucarin M (3),verrucarin J (4),roridin D (5),verrucarin L acetate (6),isororidin E (7),Roridin E (8),Roridin H (9),Roridin A (10),roridin E acetate (11),Isotrichoverrin A (12),Isotrichoverrin B (13),verrucarin A (14).Among them,compounds 2,6 and 7 were isolated from the strain for the first time.In addition,the pesticidal and herbcidal activities of the isolated 14 compounds were tested.The results showed that most compounds had positive activities on herbicidal assays and insecticidal assays.10 ppm of compounds had significant growth inhibition for Arabidopsis thaliana, 32 ppm of compounds had significant growth inhibition for Poa annua, the lethality rate of 1000 ppm in Heliothis virescens was more than 70%.The results of this study will provide a theoretical basis for the further developing trichothecenes as biological herbicides and insecticides.

Key words: Myrothecium verrucaria, chemical constituents, trichothecenes, herbcidal activity, pesticidal activity