Abstract: To study enrichment technology of chlorogenic acid and caffeic acid from Taraxacum mongolicum by macroporous resin.Determination method of chlorogenic acid and caffeic acid was established by HPLC.Preparation technology was optimized by mono factor test and orthogonal test.The optimal conditions were using XDA-1B macroporous resin,resin:Taraxacum (g:g) =1:5,the concentration of sample solution (pH3) was 40 g (Taraxacum)/L,the adsorption flow rate was 4 BV/h,The sample was eluted by water with 8 BV(flow rate was 1 BV/h) and 20% ethanol of 9 BV(flow rate was 0.5 BV/h),respectively.The content and transfer rate of chlorogenic acid and caffeic acid in enrichment were 50.32±0.74%,91.26±1.06%,37.94±0.67% and 90.14±0.93% (n=5),total content was 88.18±1.22% (n=5).The content and transfer rate was no difference by the resin with different mass (20-640 g).The resin could be repeatedly used for 4 times.The process was simple,stable,linear width,strong durability,to obtain enrichment of effective parts with high content and high transfer rate.

Key words: herba taraxaci, chlorogenic acid, caffeic acid, macroporous resin