Abstract: The effect of chlorogenic acid (CGA) and IAA/IBA on adventitious rooting of soybean hypocotyl was explored in this study.The results indicated that after treatment with 200 and 400 μmol/L CGA,rooting rate of soybean hypocotyl significantly decreased (17.8% and 26.7%,respectively),average root length significant decreased (18.7% and 35.4%,respectively);The inhibition effect of CGA on the adventitious rooting of soybean hypocotyl can be remitted by application of 2 μmol/L IAA/IBA,their rooting rate improved significantly (12.5% and 20.0%,respectively);with the increasing doses of IAA/IBA applications (10~50 μmol/L),the rooting rate significant decreased.Lignin content of soybean hypocotyl decreased in the first 24 h after treatment.The lignin content of samples suffered from 100 μmol/L CGA,increased inversely.High concentration of CGA treatment inhibited the growth of adventitious rooting of soybean.Low concentration of IAA/IBA treatment can remit the inhibition,high level of IAA/IBA treatment increased inhibition.In conclusion,CGA itself might not directly involve in organogenesis,but,might be related to a balance between IAA/IBA and lignin that control organogenesis of adventitious rooting in soybean hypocotyl.

Key words: chlorogenic acid, soybean, adventitious root, inhibition