天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (1): 41-45.doi: 10.16333/j.1001-6880.2016.1.008

• 研究论文 • 上一篇    下一篇

不同处理方法对白刺多糖抗氧化活性的影响

谢瑞1,2,魏艳霞1,2,丁玉竹1,刘燕1,2,李梦1,张继1,2,3*   

  1. 1 西北师范大学生命科学学院;2 甘肃特色植物有效成分制品工程技术研究中心;3 西北师范大学化学化工学院,兰州 730070
  • 出版日期:2016-01-29 发布日期:2016-02-01

Effects of Different Processing Methods on the Antioxidant Activity of Polysaccharide from Nitraria sibirica Pall

XIE Rui1,2,WEI Yan-xia1,2,DING Yu-zhu1,LIU Yan1,2,LI Meng1,ZHANG Ji1,2,3*   

  1. 1 College of Life Science,Northwest Normal University; 2 Bioactive Products  Engineering Research Center For Gansu Distinctive Plants,Northwest Normal University; 3 College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou 730070,China
  • Online:2016-01-29 Published:2016-02-01

摘要: 本文研究了不同处理方法对白刺多糖抗氧化活性的影响。实验通过热干、冷干、脱色等方法处理白刺多糖,从清除超氧自由基、DPPH自由基、抑制羟基自由基的产生、还原力4个指标系统地研究了不同处理方法对白刺多糖体外抗氧化活性的影响。经热干、冷干及脱色等不同方法处理得到的白刺多糖,实验所得EC50值小于0.1 mg/mL,总体来说,与经冷干得到的多糖样品相比,热干所得样品抗氧化能力有所下降,这可能是由于在热干过程中由于温度的原因引起多糖活性部分丧失,由实验数据可知经过脱色的样品对清除羟自由基(·OH)和DPPH的能力较强,而对于氧自由基(O2)的清除和还原力有所下降,相反,未经脱色的样品对氧自由基(O2)的清除能力和还原力较强,而对羟自由基(·OH)和DPPH的清除能力有所下降。结论:在体外抗氧化实验中,与热干、未脱色的多糖相比,经冷干及脱色处理得到的白刺多糖表现出较好的自由基清除能力,尤其是对超氧自由基(O2)和DPPH自由基的清除能力,因此,白刺多糖具有较好的抗氧化能力,可作为一种潜在的抗氧化剂运用于食品行业。

关键词: 多糖, 白刺, 抗氧化, 还原力

Abstract: This paper studied the effects of different processing methods on the antioxidant activity of polysaccharide from Nitraria sibirica Pall. In this experiment, polysaccharide was obtained through hot-drying,freeze-drying and decolorization. By different processing methods,the superoxide free radical (O2) and DPPH free radical (·DPPH),hydroxyl free radical (·OH) scavenging effects and reducing power of the extracted sample were measured to study the antioxidant ability of polysaccharide from N. sibirica. The results showed that the EC50 values of polysaccharide samples processed by hot-drying,freeze-drying and decolorization were all less than 0.1 mg/mL. Compared with the polysaccharide samples obtained by freeze-drying,the antioxidant ability of the samples obtained by hot-drying decreased because of high temperature resulting in the partial loss of antioxidant activity during the hot-drying process. In addition,experimental results also showed that the capability of scavenging hydroxyl free radical (·OH) in decolorization sample increased. Meanwhile,the superoxide free radical (O2) scavenging and reducing power decreased. Conversely,in the non-decolorization samples,the scavenging ability of hydroxyl free radical (·OH) and DPPH free radical (·DPPH) declined,the scavenging capacity of superoxide free radical (O2) and reducing power increased. These results indicated that the polysaccharides from N. sibirica with different processing methods showed different antioxidant activitites. It can be used as a potential antioxidant agent in food industry.

Key words: polysaccharide, Nitraria sibirica Pall;antioxidant;free radicals

中图分类号: 

S567.239