天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (增刊1): 39-45.doi: 10.16333/j.1001-6880.2022.S.006

• 研究简报 • 上一篇    下一篇

HS-SPME-GC-MS法分析薰衣草挥发性成分

陈国通1,2,马萍萍1,马锦陆3,于文蛟1,李慕春1,杨   中1*   

  1. 1新疆维吾尔自治区分析测试研究院,乌鲁木齐 830011;2新疆师范大学 化学化工学院,乌鲁木齐 830054;3新疆维吾尔自治区农药检定所,乌鲁木齐 830046 
  • 出版日期:2022-06-01 发布日期:2022-05-18
  • 基金资助:
    新疆维吾尔自治区科技支疆项目(2020E02122);天山青年计划(2018Q054);新疆维吾尔自治区天山创新团队计划(202110498)

Analysis of volatile components in Lavandula angustifolia Mill. by HS-SPME-GC-MS

CHEN Guo-tong1,2,MA Ping-ping1,MA Jin-lu3,YU Wen-jiao1,LI Mu-chun1,YANG Zhong1*   

  1. 1Xinjiang Academy of Analysis and Testing,Urumqi 830011,China;2College of Chemistry and Chemical Engineering,Xinjiang Normal University,Urumqi 830054,China; 3Institute for Pesticide Control of Xinjiang,Urumqi 830046,China
  • Online:2022-06-01 Published:2022-05-18

摘要: 为获得薰衣草在自然状态下的挥发性成分,本实验采用单因素试验和正交试验优化顶空固相微萃取-气相色谱-质谱联用技术(HS-SPME-GC-MS)条件,对代表性薰衣草品种太空蓝的常温下挥发性成分进行分析,并与水蒸气蒸馏法(steam distillation)进行了对比。结果显示,顶空固相微萃取法的优化条件为萃取时间60 min、平衡时间20 min、解吸时间5 min。该条件下,鉴定出34种化学成分,水蒸气蒸馏法鉴定出27种化学成分,主要以乙酸酯、醇、烯烃化合物为主类,其中芳樟醇、乙酸芳樟酯为主要化合物。两种方法得出的薰衣草挥发性成分无论在化学组成还是在组分的相对含量上都存在较大的差异。为薰衣草品种品质快速鉴定、质量评价及探索合理的利用方式提供了理论依据。

关键词: 薰衣草, 挥发性成分, 顶空固相微萃取, 气相色谱-质谱联用, 水蒸气蒸馏

Abstract:

In order to obtain the volatile components of Lavandula angustifolia in natural state,the conditions of headspace solid phase micro-extraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) were optimized by single factor test and orthogonal test.The volatile components of space blue,a representative variety of L. angustifolia,were analyzed at room temperature and compared with steam distillation (SD).The results showed that the optimum conditions of HS-SPME method were extraction time 60 min,equilibrium time 20 min and desorption time 5 min.Under this condition,34 chemical components were identified and 27 chemical components were identified by SD.The main compound types were acetate,alcohol and olefin compounds,among which linalool and linalyl acetate were the main compounds.The volatile components of L. angustifolia obtained by the two methods are quite different in both chemical composition and relative content.It provides a theoretical basis for rapid quality identification,quality evaluation and rational utilization of L. angustifolia varieties.

Key words:

中图分类号:  R284.2