基于与木糖进行美拉德反应的低聚壳聚糖衍生物的抗氧化性能研究

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (2): 219-223.

基于与木糖进行美拉德反应的低聚壳聚糖衍生物的抗氧化性能研究

孙涛^*，陈春红，谢晶

上海海洋大学食品学院，上海，201306

收稿日期:2010-06-09 出版日期:2011-04-25 发布日期:2011-10-14
通讯作者: taosun@shou.edu.cn
基金资助:
上海市生物医药和农业科技领域重点科技项目（08391911500）；2009年上海市优秀学科带头人计划（09XD1402000）；上海市教委重点学科建设项目（J50704）

The Maillard Reaction of Chitosan Oligosaccharide with Xylose and the Antioxidant Activity of the Chitosan Oligosaccharide Derivatives

SUN Tao^*, CHEN Chun-hong, XIE Jing

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

Received:2010-06-09 Online:2011-04-25 Published:2011-10-14

摘要/Abstract

摘要：

研究低聚壳聚糖与木糖的美拉德反应，考察了两种体系（低聚壳聚糖与木糖的质量比分别为1:1和1:3）反应过程中pH、吸光度及荧光值的变化，醇沉法提取4 h和8 h的低聚壳聚糖美拉德反应衍生物，分别为CX11-4、CX13-4、CX11-8和CX13-8。对衍生物进行红外表征和分子量测定，并研究其对羟基自由基.OH和DPPH 的清除能力以及还原能力。结果显示：壳聚糖衍生物的抗氧化能力都明显优于低聚壳聚糖，抗氧化活性顺序为CX13-4>CX11-4，CX11-8>CX13-8。可见，壳聚糖美拉德衍生物的抗氧化活性不仅与反应物的比例有关，还与反应的时间有关。

关键词: 低聚壳聚糖, 木糖, 美拉德反应, 反应时间, 抗氧化性

Abstract:

Four kinds of the chitosan oligosaccharide derivatives were prepared though Maillard reaction by heating chitosan oligosaccharide and xylose with different times (4 h and 8 h) and different proportions (the mass ratio of chitosan oligosaccharide and xylose was 1:1 and 1:3)，named CX11-4、CX13-4、CX11-8 and CX13-8，respectively. The pH，absorbance，and fluorescence were determined during the reaction. The chitosan oligosaccharide derivatives were characterized by FTIR and their molecular weight was determined by GPC. Their antioxidant activity was investigated by employing various established in vitro systems，such as hydroxyl/1，1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging and reducing power. The results showed all of the derivatives had strong antioxidant activity compared to chitosan oligosaccharide. The antioxidant activity was found to be CX13-4>CX11-4 and CX11-8>CX13-8，which indicated that the antioxidant activity of the chitosan oligosaccharide Maillard derivatives is not only related to the ratio of –NH₂and –OH but also related to the reaction times.

Key words: chitosan oligosaccharide, xylose, Maillard reaction, reaction time, antioxidant activity

孙涛，陈春红，谢晶. 基于与木糖进行美拉德反应的低聚壳聚糖衍生物的抗氧化性能研究[J]. 天然产物研究与开发, 2011, 23(2): 219-223.

SUN Tao, CHEN Chun-Hong, XIE Jing. The Maillard Reaction of Chitosan Oligosaccharide with Xylose and the Antioxidant Activity of the Chitosan Oligosaccharide Derivatives[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 219-223.

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