Abstract: To investigate the quantification method of total sugar in Hetian jujube using UV spectrophotometry and HPLC,total sugar content in Hetian jujube was determined by using phenol-sulfuric acid method and UV spectrophotometry at 489 nm.Total sugar in Hetian jujube consist three main types,including fructose,glucose,and sucrose,and their contents could be determined by HPLC,respectively.The content of total sugar in Hetian jujube was 603.30 mg/g by UV spectrophotometry,and it was 598.01 mg/g by HPLC analysis.The contents of total sugar in Hetian jujube determined by UV spectrophotometry and HPLC had small difference,which suggests that both methods are suitable to determine the total sugar in Hetian jujube.However,the HPLC method has advantages of simplicity of operation,system accuracy,high precision and reproducibility,and it is more suitable to be used for the quality control of Hetian jujube.

Key words: Hetian jujube, total sugar, HPLC, UV spectrophotometry